Figure 2From: Common variants in mismatch repair genes associated with increased risk of sperm DNA damage and male infertility FRET imaging of MLH1 and PMS2 interaction in live HEK293T cells. Images of CFP-tagged (green) and YFP-tagged (red) constructs when transiently expressed in HEK293T cells. Co-localization of co-expressed constructs is shown as yellow in overlay images. The pseudocoloured images represent FRET signals corrected for any bleed-through using the micro-FRET method (FRETc). A: Co-localization (overlay) and direct interactions (FRETc) between MLH1-CFP + PMS2 (wt)-YFP were detected in the nucleus. B: Cells co-expressing MLH1-CFP + PMS2 S775N-YFP showed good co-localization of fluorescent signals but little detectable FRETc signal in the nucleus.Back to article page