Table 1 Oligonucleotide sequences of primers used in real-time RT-PCR.
From: CD133+CXCR4+ colon cancer cells exhibit metastatic potential and predict poor prognosis of patients
Gene | Sequence (5' → 3') | |
|---|---|---|
E-cadherin | F | TGAAGGTGACAGAGCCTCTGGA |
| Â | R | TGGGTGAATTCGGGCTTGTT |
Vimentin | F | TGGCCGACGCCATCAACACC |
| Â | R | CACCTCGACGCGGGCTTTGT |
N-cadherin | F | GCGCGTGAAGGTTTGCCAGTG |
| Â | R | CCGGCGTTTCATCCATACCACAA |
β-catenin | F | AGCCGACACCAAGAAGCAGAGATG |
| Â | R | CGGCGCTGGGTATCCTGATGT |
Snail | F | CCTCCCTGTCAGATGAGGAC |
| Â | R | CCAGGCTGAGGTATTCCTTG |
GAPDH | F | TGCCAAATATGATGACATCAAGAA |
| Â | R | GGAGTGGGTGTCGCTGTTG |