Assay | Manufacturer | Method | Material | Sensitivity,% (95% CI) | Specificity,% (95% CI) | PLR | NLR | References |
---|---|---|---|---|---|---|---|---|
Amplified MTD | Gen-Probe Inc., San Diego, CA, USA | Transcription-mediated amplification | DNA from decontaminated sputum | 86.0 (74.2 to 93.7) | 99.3 (96.3 to 100.0) | 57.6 (25.5 to 129.9) | 0.1 (0.07 to 0.22) | |
COBAS® TaqMan® MTB Test | Roche Molecular Diagnostics, Pleasanton, CA, USA | RT-PCR | DNA from decontaminated sputum | 91.5 (86.9 to 96.1) | 98.7 (98.0 to 99.4) | - | - | |
79.1 | 98.2 | |||||||
artus® M. tuberculosis PCR | Qiagen, Hilden, Germany | RT-PCR | DNA from decontaminated sputum | 97.8 (93.6 to 95.5) | 85.1 (75.8 to 91.8) | 6.54 (4.0 to 10.8) | 0.03 (0.01 to 0.08) | [19] |
Loopamp® Tuberculosis Complex Detection Reagent Kit | EIKEN Chemical, Tokyo, Japan | LAMP | Untreated sputum | 88.2 (81.4 to 92.7) | - | - | - | [20] |
Amplicor MTB | Roche Molecular Diagnostics, Pleasanton, CA, USA | PCR amplification of 16S RNA | DNA from decontaminated sputum | - | - | 26.04 (17.04 to 39.80) | 0.15 (0.11 to 0.22) | [11] |
Cobas Amplicor | Roche Molecular Diagnostics, Pleasanton, CA, USA | PCR amplification of 16S RNA | DNA from decontaminated sputum | - | - | 58.59 (37.77 to 90.86) | 0.17 (0.13 to 0.22) | [11] |
LCx | Abbott Laboratories, USA, Abbott Park, IL, USA | Ligase chain reaction | DNA from decontaminated sputum | 88.9 (82.5 to 96.3) | 96.8 (95.1 to 98.5) | 26.91 (17.21 to 42.09) | 0.16 (0.12 to 0.20) | |
BD Probe Tec Direct | Becton Dickinson, Sparks, MD, USA | Strand Displacement amplification | DNA from decontaminated sputum | 77.5 (72.0 to 83.0) | 98.0 (97.1 to 98.9) | 20.11 (10.42 to 38.82) | 0.06 (0.04 to 0.10) | |
Xpert® MTB/RIF | Cepheid Inc, Sunnyvale, CA, USA | RT- PCR | Smear-positive sputum | 98.0 (98.0 to 99.0) | 99.0 (99.0 to 99.0) |  |  | |
Xpert® MTB/RIF | Cepheid Inc, Sunnyvale, CA, USA | RT-PCR | Smear-negative sputum | 75.0 (72.0 to 78.0) | 99.0 (99.0 to 99.0) |  |  |