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Table 1 Oligonucleotide primers used for quantitative RT-PCR

From: A mouse model of sitosterolemia: absence of Abcg8/sterolin-2 results in failure to secrete biliary cholesterol

Target gene

Primer sequence

Length of amplicon

GenBank accession no.

Abcg5

Forward: 5'-AGGTCATGATGCTAGATGAGC-3'

260 bp

AH011511

 

Reverse: 5'-CAAAGGGATTGGAATGTTCAG-3'

  

Abcg8

Forward: 5'-CTCCTCGGAAAGTGACAACAG-3'

190 bp

AH011518

 

Reverse: 5'-TAGATTTCGGATGCCCAGCTC-3'

  

Hmgr

Forward: 5'-CCGGCAACAACAAGATCTGTG-3'

114 bp

BB664708

 

Reverse: 5'-ATGTACAGGATGGCGATGCA-3'

  

Cyp7a1

Forward: 5'-CAGGGAGATGCTCTGTGTTCA-3'

121 bp

NM_007824

 

Reverse: 5'-AGGCATACATCCCTTCCGTGA-3'

  

Abca1

Forward: 5'-CCCAGAGCAAAAAGCGACTC-3'

89 bp

NM_013454

 

Reverse: 5'-GGTCATCATCACTTTGGTCCTTG-3'

  

Mdr2 (Abcb4)

Forward: 5'-GCAGCGAGAAACGGAACAG-3'

64 bp

NM_008830

 

Reverse: 5'-GGTTGCTGATGCTGCCTAGTT-3'

  

LXRα

Forward: 5'-GCTCTGCTCATTGCCATCAG-3'

79 bp

AF085745

 

Reverse: 5'-TGTTGCAGCCTCTCTACTTGGA-3'

  

Srebp-1c

Forward: 5'-GGAGCCATGGATTGCACATT-3'

103 bp

BI656094

 

Reverse: 5'-CCTGTCTCACCCCCAGCATA-3'

  

Srebp-2

Forward: 5'-CTGCAGCCTCAAGTGCAAAG-3'

119 bp

AF374267

 

Reverse: 5'-CAGTGTGCCATTGGCTGTCT-3'

  

Cyclophilin

Forward: 5'-AAGTTCCATCGTGTCATCAAGGAC-3'

173 bp

M60456

 

Reverse: 5'-CCATTGGTGTCTTTGCCTGC-3'

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