Figure 4

Lovastatin treatment rescues Runx2 expression in the injury site. (A)-(C) Runx2 expression analysed with radioactive in situ hybridisation on transverse sections of the drill site 7 days post injury. Representative sections of three tested animals are shown. (A')-(C') Tissue morphology visualised by bright field microscopy. (A) Intense Runx2 staining is detected throughout the bone marrow cavity (bm) and in the area occupied by newly forming trabecular bone (tb). (B) An overall decreased signal intensity in Nf1Prx1 mutants, with especially faint labelling in the area occupied by fibrous tissue (f). (C) Runx2 expression is restored within the trabecular bone formation area including the adjacent cortical defect region in lovastatin-treated mice.