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Figure 1 | BMC Medicine

Figure 1

From: Nuclear envelope structural defects cause chromosomal numerical instability and aneuploidy in ovarian cancer

Figure 1

Expression of nuclear envelope proteins in ovarian epithelial and cancer cell lines. Primary non-cancer cells and cancer cell lines were analyzed for mRNA and protein expression of nuclear envelope proteins lamin A/C, lamin B1, and emerin. (A) Total RNA was isolated and analyzed by Northern blot of primary human ovarian surface epithelial cells (HOSE166, HOSE981, HOSE663, HOSE294, and HOSE388), immortalized lines (HIO118, HIO114, HIO107, HIO105, HIO80), and ovarian cancer cells (OVCAR8, OVCAR4, OVCAR3, OVCAR5, and A2780) for the expression of lamin A/C, lamin B1, and emerin. (B) Total cell lysates from two lines of primary ovarian surface epithelial cells (HOSE18, HOSE72), an immortalized line (HIO60), and six ovarian cancer lines (OVCAR3, OVCAR5, OVCAR10, A2780, A1847, and ES2), were prepared from cultures for Western blot analysis.

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