Figure 1

Study design and analysis strategy. In the network, nodes represent omics modules (circle, metabolite module (MetM); rectangle, gene expression module (GenM)), colored according to their association with annual percentage body weight change (ΔBW; red, positive association; blue, negative association; bright color, significant, P <1.9 × 10^–4; light color, P <0.05). Edges represent partial correlations (ζ) between pairs of modules (represented by their module eigengenes), conditional on all other presented modules and the covariates age, sex, and ΔBW (solid black line, ζ >0.1; dotted black line, ζ < −0.1; solid grey line, 0.05 < ζ <0.1; dotted grey line, −0.1 < ζ < −0.05). Background color of network and boxes reflects metabolite (yellow) versus gene expression (green). GO, gene ontology; IPA, Ingenuity pathway analysis; KEGG, Kyoto Encyclopedia of Genes and Genomes; NMR, nuclear magnetic resonance; WGCNA, weighted correlation network analysis.