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Fig. 4 | BMC Medicine

Fig. 4

From: CD14hiCD16+ monocytes phagocytose antibody-opsonised Plasmodium falciparum infected erythrocytes more efficiently than other monocyte subsets, and require CD16 and complement to do so

Fig. 4

Expression of Fcγ and complement receptors on monocyte subsets and the effect of blocking antibodies on phagocytosis by individual subsets. a Expression of Fcγ and complement receptors on monocytes was determined by whole blood staining. Black bars: C, white bars: IM, grey bars: NC. Bars represent mean (sem) of MFI using blood from nine separate donors (eight for CD32b). aCD11b refers to activated CD11b defined by the epitope recognised by the CBRM 1/5 monoclonal antibody. Differences between subsets were assessed using Wilcoxon’s matched pairs signed rank test. * p <0.05, ** p <0.01. b Whole blood was incubated for 30 minutes at 4 °C with the indicated concentrations of each blocking antibody before addition of CS2-IE and determination of phagocytosis. Representative dose response curves from four independent experiments, of inhibition of phagocytosis by closed black circles), IM monocytes (open circles) and NC monocytes (open squares) are shown in the upper panels and data (mean, sem) from experiments with whole blood from three separate donors conducted using 10 μg/mL of each blocking antibody are shown in the lower panels. c Effect of blocking LFA-1 (CD11a), CR3 (CD11b) and CR4 (CD11c) on phagocytosis of IgG opsonised CS2-IE. Upper panels show dose response of the indicated blocking antibodies and lower panels show aggregate data (mean, sem from n =3 independent experiments). Symbols are the same as in a and b. C classical, IM intermediate, N non-classical

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