Genetic variation in Mycobacterium tuberculosis isolates from a London outbreak associated with isoniazid resistance

Table 2 List of genes with complete deletion in the isoniazid-resistant outbreak strains

Genes name (function)	Isolates: 02.292, 03.039, 04.018, 04.211, 04.493, 04.503, 07.116	03.313	05.177 Control
Phirv1 phage proteins (part of genomic island)	✘	✘	✓
Rv1673c (conserved hypothetical protein)	✘	✘	✓
Rv1675c (probable cAMP and macrophage regulator)	✘	✘	✓
plcD (phosphoesterase)	✘	✘	✓
Wag22 (antigen member, PE family)	✘	✘	✓
Rv1760 (possible triacylglycerol synthase)	✘	✘	✓
Rv1761c (unknown protein)	✘	✘	✓
Rv1762c (unknown protein)	✘	✘	✓
plcB (phosphoesterase)	✘	✓	✓
plcA (membrane associated phospholipase)	✘	✓	✓
PPE54 (part of PPE family)	✘	✓	✓
PE_PGRS50 (antigen member, PE family)	✘	✘	✓
PPE55 (part of PPE family)	✘	✘	✓
Rv3349c (probable transposase)	✘	✘	✓
Rv3371 (possible triacylglycerol synthase)	✘	✘	✓
Rv3486 (membrane protein, function unknown)	✘	✘	✓

Comparison is also made with possible outbreak strain 03.313 (phylogenetically related but only streptomycin monoresistant). The symbol ✘ indicates that the gene contains extensive deletions, while the symbol ✓indicates that the gene is still present. Deletions were found in 16 genes in the isoniazid-resistant tuberculosis outbreak isolates compared with the control strain 05.177. Additional comparison with another possible outbreak strain (03.313) reduced the total common deletions to 13 genes. PE and PPE: proline-glutamate (PE) and proline-proline-glutamate (PPE)

ISSN: 1741-7015