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Fig. 6 | BMC Medicine

Fig. 6

From: Abnormal epigenetic changes during differentiation of human skeletal muscle stem cells from obese subjects

Fig. 6

DNMT1, DNMT3A, and DNMT3B during differentiation of primary human myoblasts. a mRNA expression of DNMT1, DNMT3A, and DNMT3B in myoblasts, after 2 days of differentiation (intermediate) and in differentiated myotubes from six non-obese and six obese subjects. b siRNA silencing of DNMT1 in the myoblasts was confirmed after 3 and 7 days of differentiation at mRNA and protein levels. The average of Si-SCR is set to 1 at both time points for protein expression. Stain-free total protein staining was used for normalization. Representative blots are shown above the bars. Silencing of DNMT1 resulted in reduced expression of cMYC and JUNB after 3 days of differentiation (c) as well as increased expression of MYOD1 (n = 7) (d) and reduced levels of ATP (n = 3) in myotubes (day 7) (e). Data are presented as mean ± SEM, n = 4 for (b–e) if nothing else stated, except protein levels in (b) at day 3, where n = 3. *P < 0.05, **P < 0.01, ***P < 0.001

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