- Research article
- Open Access
- Open Peer Review
Effects of long-term weekly iron and folic acid supplementation on lower genital tract infection – a double blind, randomised controlled trial in Burkina Faso
- Loretta Brabin1, 15Email authorView ORCID ID profile,
- Stephen A. Roberts2,
- Sabine Gies3, 4,
- Andrew Nelson5,
- Salou Diallo6,
- Christopher J. Stewart7,
- Adama Kazienga6,
- Julia Birtles8,
- Sayouba Ouedraogo6,
- Yves Claeys9,
- Halidou Tinto10, 11,
- Umberto d’Alessandro6,
- E. Brian Faragher12 and
- Bernard Brabin13, 14
© The Author(s). 2017
- Received: 26 April 2017
- Accepted: 27 October 2017
- Published: 23 November 2017
Provision of routine iron supplements to prevent anaemia could increase the risk for lower genital tract infections as virulence of some pathogens depends on iron availability. This trial in Burkina Faso assessed whether weekly periconceptional iron supplementation increased the risk of lower genital tract infection in young non-pregnant and pregnant women.
Genital tract infections were assessed within a double blind, controlled, non-inferiority trial of malaria risk among nulliparous women, randomised to receive either iron and folic acid or folic acid alone, weekly, under direct observation for 18 months. Women conceiving during this period entered the pregnancy cohort. End assessment (FIN) for women remaining non-pregnant was at 18 months. For the pregnancy cohort, end assessment was at the first scheduled antenatal visit (ANC1). Infection markers included Nugent scores for abnormal flora and bacterial vaginosis (BV), T. vaginalis PCR, vaginal microbiota, reported signs and symptoms, and antibiotic and anti-fungal prescriptions. Iron biomarkers were assessed at baseline, FIN and ANC1. Analysis compared outcomes by intention to treat and in iron replete/deficient categories.
A total of 1954 women (mean 16.8 years) were followed and 478 (24.5%) became pregnant. Median supplement adherence was 79% (IQR 59–90%). Baseline BV prevalence was 12.3%. At FIN and ANC1 prevalence was 12.8% and 7.0%, respectively (P < 0.011). T. vaginalis prevalence was 4.9% at FIN and 12.9% at ANC1 (P < 0.001). BV and T. vaginalis prevalence and microbiota profiles did not differ at trial end-points. Iron-supplemented non-pregnant women received more antibiotic treatments for non-genital infections (P = 0.014; mainly gastrointestinal infections (P = 0.005), anti-fungal treatments for genital infections (P = 0.014) and analgesics (P = 0.008). Weekly iron did not significantly reduce iron deficiency prevalence. At baseline, iron-deficient women were more likely to have normal vaginal flora (P = 0.016).
Periconceptional weekly iron supplementation of young women did not increase the risk of lower genital tract infections but did increase general morbidity in the non-pregnant cohort. Unabsorbed gut iron due to malaria could induce enteric infections, accounting for the increased administration of antibiotics and antifungals in the iron-supplemented arm. This finding reinforces concerns about routine iron supplementation in highly malarious areas.
Trial registration number NCT01210040. Registered with Clinicaltrials.gov on 27 September 2010
- Lower genital tract infection
- Burkina Faso
Most genital tract commensals and pathogens require iron for growth and adhesion, including Gardnerella vaginalis , Trichomonas vaginalis  and Candida albicans . In contrast, most Lactobacillus species, which maintain a healthy vaginal ecosystem, do not require an iron substrate . There have been no previous randomised trials assessing whether vaginal infections are influenced by host iron status. Local iron is available from secreted lactoferrin , transudated ferritin and transferrin [6, 7], and menstrual haem iron. Host iron status could affect growth and adhesion of bacterial vaginosis (BV) , which is associated with a massive overgrowth of vaginal organisms. Dysbiosis enhances human immunodeficiency viral infectivity  and increases by two-fold the risk of preterm birth and perinatal death . Higher concentrations of transferrin in cervico-vaginal fluid were correlated with preterm birth risk in a previous study, suggesting that iron availability must be considered .
In areas where anaemia is common, the World Health Organization (WHO) recommends routine iron supplementation . It is currently unknown whether this affects bacterial profiles in the reproductive tract, especially in already iron replete women. In routine programmes, these women receive additional supplements despite there being good evidence that elevated iron stores predispose to systemic infection and inflammation . However, data are absent regarding non-malarial infection risk following iron supplementation during pregnancy , or following intermittent supplementation in menstruating women . In the context of a double blind, randomised, controlled, non-inferiority trial to assess safety of periconceptional iron supplementation in relation to malaria among young women in Burkina Faso , we assessed whether weekly iron supplementation, or baseline host iron status, affected markers of genital infection. The trial design provided two cohorts, namely a large nulliparous cohort who remained non-pregnant and received up to 18 months supplementation, and a primigravid cohort who conceived during the trial.
The trial protocol and amendments were approved by ethical review boards and regulatory authorities at each collaborating centre. This sub-study was conducted within a randomised trial of the safety of weekly iron and folic acid supplementation in young women exposed to malaria. The primary specified outcome was BV prevalence, whereas the secondary outcomes were non-BV Nugent scores, vaginal discharge and pH > 4.5. Finally, exploratory outcomes were microbiota and drug prescriptions (antibiotics, antifungals, analgesics). Additional data on the main trial and other details relevant to this paper are provided in Additional files 1, 2, 3 and 4.
Between April 2011 and January 2014 a randomised, double blind, controlled trial was conducted in rural Burkina Faso in the Nanoro Health and Demographic Surveillance System area , situated 85 km from Ouagadougou (Additional file 1). Two previous surveys in Burkina Faso indicated a BV prevalence of approximately 6–8% [18, 19]. HIV prevalence is low and reported as 1.2% among women aged 15–49 years and 0.76% among pregnant women . Women were recruited from 30 villages and individual and guardian written consents were obtained. Healthy nulliparous, non-pregnant women aged 15–24 years received either weekly ferrous gluconate and folic acid (intervention), or folic acid alone (control) as a directly observed therapy. Participation continued for 18 months for women who did not conceive or, if they became pregnant, they entered the pregnant cohort. Weekly supplementation continued until a scheduled first antenatal visit (ANC1), which was the trial primary end-point.
At enrolment demographic data and medical/obstetric histories were recorded and women were clinically examined. Height (nearest mm), weight (nearest 100 g), and mid-upper arm circumference (mm; MUAC) were measured in duplicate. A venous blood sample (5 mL) was collected for later iron biomarker assessments. Two self-taken swabs were requested for a BV slide and pH measurement. Samples were not collected during menses. Women symptomatic for T. vaginalis and BV were treated with single dose metronidazole (2 g orally), and those symptomatic for C. albicans with miconazole (200 mg intravaginally, daily for 3 days). All participants received a single dose of albendazole (400 mg) and praziquantel (1500–2400 mg according to height).
Participants were individually randomised to receive either a capsule containing ferrous gluconate (60 mg) and folic acid (2.8 mg), or an identical capsule containing folic acid alone (2.8 mg), as then recommended by WHO . This regimen was directly observed at weekly visits and continued for up to 18 months, when an end assessment (FIN) was completed and repeat vaginal swabs for BV and vaginal pH were requested. At FIN, duplicate swabs were additionally requested for preparation of vaginal fluid eluates for microbiota and T. vaginalis PCR assays. Swabs were kept cool until returned to the laboratory within 2–4 hours. A venous blood sample (5 mL) was obtained for iron biomarkers and malaria microscopy.
Women were monitored for pregnancy at weekly visits and symptoms of illness were recorded by a field worker. Symptomatic participants were directed to attend a health centre for free medical treatment for participant care, as this was required for a safety trial. Health centre staff recorded all visits, and a medically qualified researcher collated information on symptoms, clinical diagnoses and treatments. Pregnant women were instructed to attend Nanoro hospital for ANC1.
At ANC1 (primary endpoint), scheduled at 13–16 weeks’ gestation, a venous blood sample (5 mL) was obtained for iron biomarkers and malaria microscopy, and self-taken vaginal swabs requested as for the non-pregnant cohort. An ultrasound examination was completed to date gestational age. Symptomatic women were treated for BV and T. vaginalis with metronidazole 500 mg orally twice daily for 7 days, for C. albicans with intravaginal miconazole 200 mg daily for 3 days, and for N. gonorrhoeae and C. trachomatis with ceftriaxone 250 mg intramuscularly once and amoxycillin 500 mg orally thrice daily for 7 days for suspected cervical infection. Women were encouraged to deliver at their closest health centre. The study provided free obstetric care.
BV slides were fixed, Gram stained, air dried and forwarded to the Microbiology Laboratories, Central Manchester University Hospital NHS Trust, UK, for Nugent scoring . Approximately 10% of slides, plus indeterminate slides, were read in duplicate. Nugent scores of 7–10 indicated BV, 4–6 intermediate, and 0–3 normal flora. Gram stains were available for 80.5% of women at enrolment, 76.5% at FIN, and 94.6% at ANC1, with no differences between trial arms.
Each tube containing a swab for vaginal eluate was processed on laboratory arrival when 5 mL of PBS was added to the tube and shaken at high speed (5 minutes), before pipetting and freezing (–20 °C). Vaginal eluates were air freighted on dry ice to the University of Northumbria, UK.
Blood films were stained with Giemsa and read independently by two qualified microscopists and, in the case of discordant results, by a third reader.
At the Nanoro Research Laboratories, ferritin, indicative of iron stores, and serum transferrin receptor (sTfR), indicative of functional iron deficit, were measured in duplicate by ELISA (Spectro Ferritin S-22 and TFC 94 Transferrin Receptor, RAMCO Laboratories Inc., Texas) and C-reactive protein (CRP) by ELISA (EU59131, IBL International, GMBH, Hamburg). Definitions of iron deficiency were (1) adjusted ferritin (adjFE) allowing for inflammation, ferritin < 15 μg/L if CRP < 10 μg/mL, or ferritin < 70 μg/L if CRP ≥ 10 μg/mL; or (2) a ratio of sTfR μg/mL to log10 ferritin > 5.6, , which assesses both stored and functional iron and is possibly less affected by inflammation.
Microbiota/T. vaginalis qPCR
DNA was extracted from vaginal eluates using the PowerLyzer Power Soil kit (MoBio, SD, USA) with the following modifications. A 250-μL aliquot of vaginal eluate was combined with 500 μL of bead solution added to the bead tube and processed following the manufacturer’s instructions. DNA extraction negative controls were processed for each kit and sequenced alongside test samples. Bacterial profiling of the variable region 4 (V4) of the 16S rRNA gene was performed by NU-OMICS (Northumbria University) based on the Schloss wet-lab MiSeq procedure , and T. vaginalis by qPCR  (Additional file 2).
The sample size was determined from formal power calculations for the malaria trial endpoints . Analyses excluded women who remained non-menarcheal throughout the trial and any who became menarcheal within 6 months of FIN when menses may have been irregular. The primary analyses were comparisons of BV (Nugent > 6) risk at ANC1 and at FIN (menarcheal) by arm on an intention to treat basis using a binomial model with a log link, and baseline infection and use of antibiotics in the 3 months prior to assessment as covariates. Other infection markers were analysed similarly. Nugent scoring gave a 3-level classification and each level was compared to the remaining levels. Intention to treat analyses of iron deficiency used a similar approach with no covariates.
Associations between baseline infections and dichotomised iron deficiency markers were similarly analysed, but with no covariate for antibiotic use, as free study prescriptions had not commenced. The analysis adjusted for MUAC, a surrogate for nutritional status. Associations between baseline infection indicators and continuous biomarkers followed similar binomial models, with log-transformed values scaled by their standard deviations to give comparable risk ratios across markers. The P values for multiple markers were adjusted for multiple testing using the false discovery rate method .
Receipt of antibiotics, antifungals and analgesics was classified by clinical indication. Poisson regression models were used to estimate incidence ratios and to test for differences between arms, using number of visits between enrolment and assessment as an exposure time offset. Incidence rates are presented per 50 visits as this approximated the mean number of visits prior to follow-up assessment. For separate indications, P values were adjusted using the false discovery rate approach.
The statistical methods for analysis and visualisation of microbiota communities are described in Additional file 2. Multinomial regression models were used to identify if Community State Type (CST) was associated with trial arm, pregnancy, iron deficiency or infection markers.
Baseline characteristics by trial arm of participants who subsequently became pregnant or remained non-pregnant during follow-up
N = 258
N = 220
N = 766
N = 783
Age, years, mean (SD)
Ethnic group, n (%)
Occupation,b n (%)
Education, n (%)
Literate, n (%)
Reproductive history and infections, n (%)
Menarche age, mean (SD)
Ever had sex
Vaginal pH ≥ 4.5
Nutrition and iron biomarkers, n (%)
BMI, kg/m2, mean (SD)
MUAC, cm, mean (SD)
Iron deficient (adjFE)
Iron deficient (ratio sTfR/log ferritin)
Adherence to treatment
DOT,c mean (SD)
Infections and iron status at baseline assessment
Baseline host iron status using two definitions of iron deficiency and infection markers (n = 1673, excluding 281 non-menarcheal)
RR (95% CI)
RR (95% CI)
pH ≥ 4.5
sTfR/log ferritin ratio > 5.6
pH ≥ 4.5
Lower genital tract infection in the pregnant cohort
Prevalence of iron deficiency (adjFE) did not differ significantly between trial arms (6.9% (iron) and 12.7% (control); RR 0.54, 95% CI 0.27–1.1, P = 0.12) or based on the sTfR/log ferritin ratio (11.1% (iron) and 12.7% (control); RR 0.88, 95% CI 0.48–1.61, P = 0.73).
Lower genital tract infection indicators in pregnant (ANC1) and non-pregnant (FIN) cohorts by trial arm
RR (95% CI)
RR (95% CI)
N = 315
T. vaginalis f
pH ≥ 4.5
N = 877
T. vaginalis f
Lower genital tract infection in the non-pregnant cohort
Prevalence of iron deficiency (adjFE) was 9.0% (iron) and 11.0% (control) (RR 0.82, 95% CI 0.55–1.22, P = 0.37) and, based on sTfR/log ferritin ratio, it was 20.1% (iron) and 21.2% (control) (RR 0.95, 95% CI 0.73–1.23, P = 0.74).
No prevalence difference was observed for BV (13.5% iron vs. 12.1% control, RR 0.86, 95% CI 0.56–1.34, P = 0.51), intermediate flora (10.3% vs. 7.9%, P = 0.47), or T. vaginalis (5.6% vs. 4.2%, P = 0.43) (Table 3). Vaginal discharge was more frequent in iron-supplemented women (11.5% vs. 6.9%, P = 0.026). This difference would be considered non-significant allowing for the number of secondary outcomes tested. Abnormal discharge occurred in 9% of those with BV and 18% with T. vaginalis.
Comparison of non-pregnant and pregnant cohorts
Cross-sectional BV prevalence was higher in non-pregnant women at FIN (12.8%) than at ANC1 (7.0%) (RR 1.8, 95% CI 1.2–2.9, P = 0.011, adjusted for antibiotic use), but T. vaginalis prevalence was lower (4.9%) (RR 0.38, 95% CI 0.24–0.59, P < 0.001). P. falciparum prevalence was 54% at ANC1 and 41% at FIN, despite free access to anti-malarial treatments.
Health Centre treatment courses prescribed to pregnant and non-pregnant cohorts by trial arm from enrolment to ANC1 (pregnant) or FIN (non-pregnant)
Number of treatments
Mean treatments per person
Mean treatments per 50 visitsa
Incidence ratiob (95% CI)
n = 163
n = 152
Visits = 6907
Visits = 6235
n = 426
n = 451
Mean number of health centre antibiotic treatments in non-pregnant cohort by infection diagnosis and trial arm from enrolment to FIN
Iron per 50 visits
n = 26,473
Control per 50 visits
n = 27,633
Community state type distribution in pregnant and non-pregnant (menarcheal) cohorts by trial arm, iron deficiency and infection status
Iron deficiency, Adjusted ferritin
Ratio sTfR/log ferritin
High pH ≥ 4.5
Antibiotics within previous three months
In this young population, reported sexual activity was low at recruitment but its commencement carried a substantial risk of immediate pregnancy, a sexually transmitted infection (T. vaginalis), an unstable microbiota and malaria, which was more frequent in primigravidae. Evidence on effects of intermittent iron supplementation on infectious disease outcomes is scarce and unclear, with few studies and small sample sizes . This randomised, controlled, double blind trial found no evidence that 60 mg iron/2.8 mg folic acid supplements offered weekly, increased the risk of BV, T. vaginalis, or CST IV microbiota in either the pregnant or non-pregnant cohort. A two-fold increased use of antibiotics for treatment of gastrointestinal infections, with increased use of anti-fungals for lower genital infection in the non-pregnant iron supplemented cohort, raises new concerns about the safety of weekly iron supplements for young women. Nutritional status, reflected by MUAC, was an effect modifier for BV infection. This result adds to a growing body of evidence of an association between diet and BV, for which there is currently no clear explanation .
A major strength of this study was its large cohort of non-pregnant peri-menarcheal adolescents, as well as a concurrent pregnancy cohort . Some loss to follow-up occurred due to movement outside the study area following marriage, unwanted pregnancy or, if unmarried, working for relatives living elsewhere. Trial participants were young, nulliparous girls, with low prevalence of iron deficiency at recruitment. Accurate estimation of iron deficiency prevalence is influenced by effects of inflammation on indices such as ferritin. A recent meta-analysis in women of reproductive age experiencing inflammation estimated the difference of depleted iron stores between adjusted and unadjusted ferritin values as 2–8 median percentage points, dependent on the adjustment method . We used a high ferritin cut-off of < 70 μg/L for iron deficiency in women with CRP ≥ 10 μg/mL, allowing for inflammation, as well as the lower cut-off < 15 μg/L with CRP < 10 μg/L to include those without inflammation. CRP concentration increases in early pregnancy, which was accommodated by using the CRP < 10 μg/L cut-off. Although prevalence of iron deficiency in absolute terms varied according to the CRP cut-off used, sensitivity analyses showed that trends and trial arm differences in iron deficiency were similar irrespective of CRP level. We additionally used the sTfR/log10 ferritin ratio > 5.6, which is derived from an intermediate ferritin cut-off of < 30 μg/L . We considered this provided a reasonable estimate of iron deficiency. Sensitivity analysis of alternative ferritin thresholds for defining iron deficiency, adjusting for inflammation, gave very similar results.
Despite good supplementation adherence over an 18 month period, facilitated by weekly direct observation, there was no significant improvement in systemic iron biomarkers. The absence of an association between iron supplementation and BV or T. vaginalis prevalence could relate to the lack of intervention efficacy in reducing iron deficiency. At baseline, iron-replete participants were less likely to have normal vaginal flora, which suggests host iron status is reflected in the mucosa. Following supplementation, an increased prescription for fungal infections was also observed inferring an iron-fungal infection interaction . Antibiotics used for treatment of enteric infections may have altered lower genital tract bacterial profiles. Cessation of menses with pregnancy would also reduce iron availability to haem-utilising pathogens.
Chronic inflammation from malaria, which was frequent, as well as other infections, would increase hepcidin release, restricting iron absorption. In Beninese women with afebrile P. falciparum parasitaemia, dietary iron absorption was reduced by approximately 40% with infection . In the non-pregnant cohort, a significantly higher number of antibiotic-treated gastrointestinal infections – predominantly diagnosed as dysentery, typhoid, and intestinal parasitosis – occurred in supplemented women, possibly related to unabsorbed iron becoming available to colonic gut microbiota . Although some misclassification of infection categories would arise due to a lack of laboratory confirmation, this should not alter findings by trial arm. Increased gut inflammation and pathogenicity following iron supplementation, or fortification, is reported in children exposed to high infection pressure in Ghana  and Kenya , and increased risk of bloody dysentery and respiratory infection in Pakistan . This is the first report in young women to show increased enteric symptoms requiring antibiotic treatments following iron supplementation. Concurrent infection with non-typhi salmonella and P. falciparum should be considered . Increased use of antifungal prescriptions in the iron-supplemented non-pregnant cohort is of interest because, both in the gut and vaginal mucosa, C. albicans is a normal commensal, competing with other organisms for nutrients, and increased fungal virulence due to iron malabsorption could be anticipated. In addition, disruption to normal bacterial gut populations due to tetracycline treatment can result in candida overgrowth, reducing colonisation resistance . The intestinal tract is considered as a reservoir of infection leading to recurrent vaginal candidiasis based on the correspondence between vaginal and stool samples .
Self-taken vaginal swabs proved acceptable, providing samples for gram stains, vaginal eluates and microbiota assays. The absence of differences in microbiota CST categories by trial arm is consistent with no supplementation effect on BV or T. vaginalis. BV was associated with CST IV, and both had lower prevalence in pregnant than non-pregnant women . This pregnancy difference is probably related to hormonal factors , although we cannot exclude the possibility that it is mediated by differences in other characteristics between those who became pregnant and those that did not. In marked contrast, T. vaginalis prevalence, also associated with CST IV, was three-fold higher at ANC1 compared to non-pregnant women at FIN, probably due to more regular sexual activity with male partners untreated for this sexually transmitted infection. Interactions between BV and T. vaginalis are poorly understood  but, under experimental conditions, T. vaginalis is associated with reduced populations of Lactobacillus spp, but not BV spp . Co-infection in early pregnancy may hinder transition to stable microbiome communities, such as CST I, which is associated with better pregnancy outcomes . Clinical outcomes may depend on initial host iron status, as experimental studies have shown altered inflammatory responses, higher abundance of Bifidobacteriaceae and Lactobacillaceae, and prolonged intestinal nematode survival in animals receiving iron-deficient diets .
At baseline, elevated vaginal pH values (≥4.5) were observed in iron-replete participants and were positively associated with plasma ferritin (P = 0.015). High vaginal pH may be attributable to normal pubertal changes , young age, irregular menses , and factors impacting on lactobacilli or other lactic acid producing microbes , but also to genital tract infection. The reason in this study for the significant association of high pH and more disturbed flora in adolescents with better iron stores before supplementation is unknown and warrants further investigation.
There was no evidence that weekly iron increased the risk of BV or T. vaginalis infections, but the intervention was considered poorly absorbed as systemic iron biomarkers were not significantly changed. Iron supplementation did result in more gastrointestinal morbidity, leading to increased antibiotic prescription and genital tract infection requiring antifungal treatments in the non-pregnant cohort. This is a concern since recently revised WHO recommendations are shifting in favour of providing intermittent daily iron . A daily regime could be more likely to exacerbate gut microbes and/or increase antibiotic/antifungal prescriptions. Since malaria is the most likely cause of iron malabsorption in the gut, iron supplementation studies in malaria endemic areas need to profile enteric and vaginal infections, as well as antibiotic use.
We gratefully acknowledge the contribution and support of participating women, local communities, the memory of his late Majesty Naaba Tigré of Nanoro, study teams, female field assistants, and nurses, midwives, supervisors, doctors and staff of Nanoro Health District and peripheral Health Centres, St Camille hospital. Additionally, we thank the laboratory assistance of Greg Harper and Marc Tahita; quality control staff at G&G Food Supplies Ltd, East Grinstead, West Sussex, UK; members of the Data Safety and Monitoring Board; Chris Roberts, Chair, University of Manchester, UK; Patrick van Rheenen, University of Groningen, Netherlands; Marleen Boelaert and Veerle Van Lerberghe, Institute of Tropical Medicine, Antwerp, Belgium; and Professor Stephen Cummings, Associate Academic Dean Health and Life Sciences, who facilitated microbiota analyses at Northumbria University. We are grateful to Raffaella Ravinetto and Céline Schurmans of the Clinical Trials Unit, Institute of Tropical Medicine, Antwerp, and Isidore Traore for their efforts on trial monitoring activities.
This work was supported by the National Institutes of Health (Grant Number U01HD061234-01A1; Supplementary -05S1 and -02S2), the National Institute of Child Health and Human Development, and the National Institutes of Health Office of Dietary Supplements. The grant covered the cost of all field work, including local salaries, and funded the Tropical Institute in Belgium for the salary costs of SG, who was stationed in Burkina Faso. The University of Manchester received part-time salary costs for statistical analysis by SR; JB received pro rata costs for slide reading. The University of Northumbria was given a sub-contract for microbiota profiling. Salary costs of LB, BJB and YC were borne by their own academic institutions. The Scientific Advisory Committee was reimbursed for travelling expenses.
The contents of this publication are solely the responsibility of the authors and do not necessarily represent the views of the funding organisation, which played no role in data analysis.
Availability of data and materials
The trial protocol was reported in the Lancet (www.thelancet.com/protocol-reviews/10PRT-6932). Until placed in a public repository, data relating to the current study can be requested from the corresponding author and will be made available following an end-user data agreement and sponsor approval.
BB was the Principal Investigator for the main RCT on iron supplementation and is accountable for all aspects of the work; he co-wrote the paper. LB conceived the study and wrote the paper. SR was co-author and study statistician. SG was the clinical and field co-ordinator for the research. Iron assays were conducted in Burkina Faso by SD, microbiome by AN and CS at Northumbria University, UK, and BV gram stains by JB at Manchester, UK. AZ, SO and YC were responsible for data management and safety monitoring. BF was the main trial statistician. UDA and HT were members of the Scientific Advisory Committee and reviewed the paper. All authors read and approved the final manuscript.
Ethics approval and consent to participate
The clinical protocol was approved by the Liverpool School of Tropical Medicine, UK, Research Ethics Committee (LSTM/REC), the Institutional Review Board of the Institute of Tropical Medicine, Antwerp, Belgium (IRB/ITM), the Antwerp University Hospital Ethics Committee (EC/UZA), the Institutional Ethics Committee of Centre Muraz (Comité d’Ethique Institutionnel du Centre Muraz, CEI/CM), and the National Ethics Committee (Comité Ethique pour la Recherche en Santé, CERS) in Burkina Faso.
Prior to enrolment, the study team visited each village to inform village elders and senior women about trial objectives and for permission to invite young women to take part. Informed consent with right to withdraw (signature or thumb print) was granted by each participant, or by her appointed guardian if a minor or married at recruitment, and repeated later by participants continuing to be followed in the pregnant cohort.
Consent for publication
Participants provided written informed consent for publication of research results.
The authors declare that they have no competing interests.
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