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Table 2 Phosphorylated hepatic proteins with correlations with fibrosis and a higher percentage of collagen deposition in livers of NAFLD patients

From: An exploratory study examining how nano-liquid chromatography–mass spectrometry and phosphoproteomics can differentiate patients with advanced fibrosis and higher percentage collagen in non-alcoholic fatty liver disease

Endpoint

Protein intensity values (mean)

MITOGENESIS

Fibrosis (stage ≥ 2)a,b

Fibrosis (stage < 2)a,b,c

Percentage collagen ρc,d

RTKS and LIGANDS

 c-Kit Y703

12,023.3253

8509.7594**

0.2727*

 EGFR Y1045

7752.7248

5367.3954**

0.3061*

 ErbB2 HER2 Y1248

13,638.4722

10,163.0936**

0.2544*

 Met Y1234/1235

8394.0971

5442.5366***

0.4082***

 Ret Y905

4840.5597

2796.3384**

0.2564*

 Ron Y1353

11,455.9458

8217.8116**

0.2564*

DOWNSTREAM SUBSTRATES

 CrkII Y221

11,296.7507

7283.2336**

0.2844*

 c-Myc

16,583.2182

13,624.0484*

0.2705*

 ERK 1/2 TOTAL

27,683.0657

22,584.3185**

0.3574**

 FRS2 alpha Y436

6745.9092

4569.8235*

0.3382**

 IRS 1 S612

6603.2819

4223.5915*

0.2756*

 PKA C T197

13,849.3962

11,729.8932*

0.2968*

 Src Family Y416

3488.0194

1874.4209**

0.2631*

SURVIVAL

 AKT T308

19,966.2896

11,089.2265**

0.3763**

 AKT TOTAL

15,739.0600

10,127.1820**

0.3282**

 ASK1 S83

2072.4277

1209.4175**

0.2563*

 Ephrin A3 Y779/A4 Y779/A5 Y833

10,051.2853

9008.6468*

0.3179*

 PDK1 S241

13,526.9204

10,692.6456**

0.2990*

 PI3K p85 Y458 p55 Y199

5930.4957

4023.6630**

0.4243***

 PTEN

26,544.6698

22,904.4300*

0.2628*

 PTEN S380

12,498.2321

7720.4331***

0.2677

INFLAMMATION/IMMUNE FUNCTION

 Zap 70 Y319/Syk Y352

8322.9034

4839.8932***

0.3189*

 cPLA2 S505

11,697.1946

8108.4437**

0.2861*

 PLCgamma1 Y783

11,995.7606

8488.3315**

0.2824*

 Stat4 Y693

8407.7191

5859.6088**

0.3010*

 Stat6 Y641

7840.7122

4793.4883***

0.3226*

 Tyk2 Y1054/1055

8760.8030

5060.9800***

0.2955*

 p38 MAPK T180/Y182

8093.9200

6807.1800

0.3901**

APOPTOSIS

 Bak

20,974.0624

18,147.0249**

0.3262**

 Survivin

11,593.7421

8882.5924***

0.3180*

MOTILITY AND CELL ADHESION

 Cofilin S3

11,887.6509

2171.5929***

0.3369**

 FAK Y576/577

7300.4566

5303.6335*

0.3382**

OTHERS

 ALDH

21,453.9429

26,006.4687**

−0.3009*

 IL-10

1731.1300

1311.5200

0.5539***

  1. a Proteins in liver tissue, detected using RPMA, that were expressed at higher and significant level in patients with fibrosis ≥ 2 compared with fibrosis < 2
  2. b Non-parametric Kruskal–Wallis H test was used to identify the significantly differentially expressed proteins between the two groups
  3. c Protein level correlation with % collagen tested using Spearman rank correlation test
  4. d Correlation coefficient (ρ) describes the strength and direction of association between proteins and % collagen
  5. p values < 0.05 are considered significant; *p < 0.05, **p < 0.01, ***p < 0.001