Fig. 4
Cytokine responses to P. falciparum 7G8 in study participants inoculated with a single dose of a 3 × 107P. falciparum 7G8 pRBC treated with 50 nM (group A) or b 200 nM (group B) TF-A. Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples collected at different time points post-inoculation and cryopreserved. Following thawing, PBMCs were incubated with parasitised red blood cells (pRBC) or unparasitised red blood cells (uRBC) for 7 days. Eighteen hours before the end of the culture period, culture supernatants were collected, pooled (n = 3) and used in cytokine bead arrays to quantify the level of cytokines produced in response to P. falciparum 7G8 pRBCs by flow cytometric analysis. Delta cytokine indicates that responses to pRBC were corrected against responses to uRBC