Fig. 3

Intermediate and prolonged effects of cisplatin exposure on germ cell populations in the human foetal testis. AP2γ⁺ gonocytes (brown) and MAGEA4⁺ (pre)spermatogonia (blue) protein expression in the human foetal testis 72 h (a, b) and 240 h (f, g) following exposure to vehicle or cisplatin (0.5 μg/ml). Scale bars represent 100 μm (or 50 μm for insets). Dotted lines outline seminiferous tubules. Quantification of germ cell subpopulations in the human foetal testis 72 h (c–e) and 240 h (h–j) following exposure to vehicle (VEH) and cisplatin (CIS; 0.5 μg/ml). At 72 h post-exposure to cisplatin, gonocytes and total germ cells were significantly reduced, whilst (pre)spermatogonia were unaffected. At 240 h post-exposure, a decrease in all germ cell populations was observed. Data analysed using two-way ANOVA. *p < 0.05, **p < 0.01 and ****p < 0.0001. Values shown are means ± SEM and each set of data points represents an individual foetus (n = 8–14)