Fig. 6

Effects of cisplatin exposure on human prepubertal testis. a t-SNE plot of cell populations obtained during single-cell sequencing of testicular tissue of a 7-year-old male. b Violin plots for normalised expression of (pre)spermatogonium (MAGEA4) and SSC (UTF1) populations in testicular tissues from 1-, 7- and 11-year-old (yo) males. Red dots represent mean expression. Effect of cisplatin (CIS, 0.5 μg/ml) exposure on MAGEA4⁺ spermatogonia (blue) and UTF1⁺ (green) SSCs in the human prepubertal testis 24 h (c–e) and 96 h (f–h) after exposure compared to vehicle (VEH) control. A significant decrease in spermatogonia and SSCs was observed at 96 h post-cisplatin exposure. Data analysed using two-way ANOVA. *p < 0.05. Values shown are means ± SEM and each set of coloured data points (blue and red—1 year, orange—8 years, green—11 years, purple—12 years) represents an individual patient (n = 3–5). c, f Arrows—spermatogonium (MAGEA4⁺), arrowheads—SSC subpopulation (MAGEA4⁺/UTF1⁺)