Figure 2From: Human amniotic fluid stem cell injection therapy for urethral sphincter regeneration in an animal model Myogenic differentiation of hAFSCs in vitro. (A) Viability assay for cells cultured in three different myogenic induction media for 14 days. Cell viability in CM was 2.7- (P < 0.0001) and 1.58- (P < 0.0001) fold higher than that of cells cultured in medium containing 5-azaC or TGF-β respectively. (B) Real-time PCR analysis of expression of myogenic lineage markers in three different media at Days 3 and 7. The expression of early myogenic differentiation markers (PAX7 and MYOD) was dominant at Day 3 and the expression of the mid to late myogenic marker (DYSTROPHIN) became dominant at Day 7 (**P < 0.01; *P < 0.05). (C) Double staining using primary myogenic antibodies and DAPI through ICC analysis (200×) at Day 7. Cells were strongly positive for MYOD and DESMIN. Ctrl(+), positive control C2C12 cells; Ctrl(-), negative control with human fibroblasts.Back to article page