Figure 1

Transcription activity analysis of the -448G>A polymorphism of DNMT3A promoter. Chinese hamster ovary cells were transiently transfected with luciferase reporter constructs. The pGL3-basic plasmid lacking promoter sequence was used as a negative control. The data represents mean -- standard deviation calculated from four independent experiments performed in triplicates and are expressed as a percentage of the -448A allele activity. The difference between the -448G and -448A constructs was significant at P < 0.001 (t-test; n = 6).