Figure 1

Schematic representation of lymph nodes sectioning protocol for immunohistochemistry (solid line) and molecular (dotted line) analyses. (A) Non-metastatic lymph nodes: all sections at different levels were used for RNA extraction and subsequent molecular analysis (arrows). (B) Metastatic lymph nodes: only sections of the positive level at IHC analysis were used for RNA extraction and subsequent molecular analysis (arrow). The leftover material (regions marked with “*”) removed from each interval of 50 μm was collected and used in the analysis of the leftover material.