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Table 2 Sensitivity and specificity values of microRNAs evaluated in discriminating metastatic and non-metastatic lymph nodes in FFPE and FNA biopsies from lymph node samples

From: Accuracy of microRNAs as markers for the detection of neck lymph node metastases in patients with head and neck squamous cell carcinoma

microRNA

 

Sensitivity

Specificity

Cutoffa

Metastaticb

Macrometastases

Micrometastases

Isolated tumor cells

Non-metastatic

% (95% CI) (n)

% (95% CI) (n)

% (95% CI) (n)

% (95% CI) (n)

% (95% CI) (n)

FFPE samples

miR-200a

5.96

76.0 (54.5–89.8) (19/25)

94.4 (70.6–99.7) (17/18)

40.0 (7.3–82.9) (2/5)

0 (0–80.2) (0/2)

100 (71.7–100) (13/13)

miR-200c

2.33

88.0 (67.7–96.8) (22/25)

100 (78.1–100) (17/18)

80.0 (29.9–98.9) (4/5)

0 (0–80.2) (0/2)

100 (71.7–100)) (13/13)

miR-203

1.96

100 (83.4–100) (25/25)

100 (78.1–100) (17/18)

100 (46.3–100) (5/5)

100 (19.8–100) (2/2)

100 (71.7–100) (13/13)

miR-205

1.54

100 (83.4–100) (25/25)

100 (78.1–100) (17/18)

100 (46.3–100) (5/5)

100 (19.8–100) (2/2)

100 (71.7–100) (13/13)

FNA samples classified by cytology

miR-203

10

100 (91.5–100) (42/42)

100 (91.5–100) (42/42)

N/A

N/A

100 (94.9–100) (71/71)

miR-205

10

100 (91.5–100) (42/42)

100 (91.5–100) (42/42)

N/A

N/A

100 (94.9–100) (71/71)

FNA samples classified by histology

miR-203

10

92.9 (80.5–98.4) (68/71)

100 (89.3–100) (68/68)

0 (0) (0/2)

0 (0) (0/1)

100 (94.9–100) (71/71)

miR-205

10

92.9 (80.5–98.4) (68/71)

100 (89.3–100) (68/68)

0 (0) (0/2)

0 (0) (0/1)

100 (94.9–100) (71/71)

  1. FFPE, Formalin-fixed paraffin embedded; FNA, Fine-needle aspiration; PPV, Positive predictive value; NPV, Negative predictive value; AUC, Area under the ROC curve; CI, Confidence interval; N/A, Not applicable. aThe cutoff values for FFPE samples were determined according to the Youden index (value in which the difference between sensitivity and 1-specificity is maximum) obtained from the ROC curves. bThe ‘metastatic’ group comprises all cases with positive lymph nodes (macrometastases, micrometastases, or isolated tumor cells).