Genetic variation in Mycobacterium tuberculosis isolates from a London outbreak associated with isoniazid resistance

Satta, Giovanni; Witney, Adam A.; Shorten, Robert J.; Karlikowska, Magdalena; Lipman, Marc; McHugh, Timothy D.

doi:10.1186/s12916-016-0659-6

Table 1 List of Mycobacterium tuberculosis clinical strains from the selected cluster RFL15

From: Genetic variation in Mycobacterium tuberculosis isolates from a London outbreak associated with isoniazid resistance

Type	Isolate	Lineage (clade)	Resistant to	Sensitive to	Polymorphism for INH	Relative fitness to H37Rv
Cluster RFL15	02.113	Euro American (Cameroon)	-	H,R,Z,E,S	None detected	0.96
	02.292	Euro American (Cameroon)	H	R,Z,E,S	*inhA C → T*	0.88
	02:302^a	Euro American	S	H,R,Z,E	None detected	1.05
	03.013	Euro American (Cameroon)	S	H,R,Z,E	None detected	0.89
	03.039	Euro American (Cameroon)	H	R,Z,E,S	*inhA C → T*	0.82
	03.303^a	Euro American	H	R,Z,E,S	inhA C → T	0.92
	03.313	Euro American (Cameroon)	S	H,R,Z,E	None detected	0.98
	04.018	Euro American (Cameroon)	H, R, clari, ethi	Z,E,S	*inhA C → T*	0.75
	04.194	Euro American (Uganda)	H	R,Z,E,S	katG S315T	0.96
	04:198^a	Euro American	H	R,Z,E,S	inhA C → T	0.98
	04.211	Euro American (Cameroon)	H	R,Z,E,S	*inhA C → T*	0.93
	04.493	Euro American (Cameroon)	H	R,Z,E,S	*inhA C → T*	1.01
	04.503	Euro American (Cameroon)	H	R,Z,E,S	*inhA C → T*	0.94
	05.046	Euro American (Cameroon)	-	H,R,Z,E,S	None detected	1.03
	07.116	Euro American (Cameroon)	H, ethi	R,Z,E,S	*inhA C → T*	0.99
	07:118	Euro American (Cameroon)	H	R,Z,E,S	katG S315T	0.82
H-resistant control	05.177 (Control strain 1)	Euro American (Uganda)	H	R,Z,E,S	inhA C → T	0.92
H-sensitive control	05.094 (Control strain 2)	Euro American (Uganda)	-	H,R,Z,E,S	None detected	0.88
H-sensitive control	04.011 (Control strain 3)	East African Indian (Delhi/CAS)	-	H,R,Z,E,S	katG R463L (but no resistance)	0.81
	Mutation rate - outbreak strains			Mutation rate - control strains
	1.3 × 10⁻⁸ × cell division			1.3 × 10⁻⁸ × cell division

^ano whole-genome sequence available as unable to extract enough DNA
The first two digits in the isolate number indicate the year of isolation, for example, 02:113 was isolated in 2002. Sensitivities were based on phenotypical testing (abbreviations: Clari clarithromycin, Ethi ethionamide, H isoniazid, R rifampicin, Z pyrazinamide, E ethambutol, S streptomycin). Only the genetic mutations conferring isoniazid resistance are reported for simplicity. Strains in bold are closely related on further phylogenetic analysis (see phylogenetic tree)

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ISSN: 1741-7015

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