Fig. 2

HRCA-PDOs retained the histopathological features, mutational fingerprint and molecular characteristics of primary tissues. A H&E staining, bright-field images, and E-cadherin (E-Cad) immunofluorescence staining on primary adenomas and/or organoids from patients 1 and 2. Scale bar, 50 μm. B Immunofluorescent staining of mucin2 on HRCA-PDOs from patients 4, 14, 15, and 20 labeled by organoids 4, 14, 15, and 20. Scale bar, 50 μm. C Immunohistochemical staining of Ki67 and c-Myc on primary adenomas and HRCA-PDOs. Scale bar, 100 μm. D The comparison of mutation landscapes of adenomas from four patients P1–4 (P1, P2, P4, and P5 in the Table S1) and the corresponding HRCA-PDOs 1–4 (O1–O4) were displayed. The type of genetic alteration (noted by color code) was displayed for the commonly mutated genes. E Concordances of somatic mutations were analyzed for paired adenomas and HRCA-PDOs. Bar graph represents the proportion of coding alterations that are concordant between the primary adenoma and the corresponding organoid culture and those that are found only in organoid or primary adenoma specimen