Small volume bone marrow aspirates with high progenitor cell concentrations maximize cell therapy dose manufacture and substantially reduce donor hemoglobin loss

Table 2 Comparison of MSC doses that can be manufactured with small volume aspirates and large volume aspirates

Number of MSC doses that can be manufactured from a given BM volume	child @ 10 kg BW target dose 1 × 10⁶ MSCs/kg BW		child @ 10 kg BW target dose 2 × 10⁶ MSCs/kg BW
BM volume (mL)	Small volume aspirate	Large volume aspirate	Small volume aspirate	Large volume aspirate
50	294	193	147	97
100	587	386	294	193
500	2937	1931	1468	966
	% dose production compared to large volume		% dose production compared to large volume
	152.04		152.04
Number of MSC doses that can be manufactured from a given BM volume	adult @ 80 kg BW target dose 1 × 10⁶ MSCs/kg BW		adult @ 80 kg BW target dose 2 × 10⁶ MSCs/kg BW
BM volume (mL)	Small volume aspirate	Large volume aspirate	Small volume aspirate	Large volume aspirate
50	37	24	18	12
100	73	48	37	24
500	367	241	184	121
	% dose production compared to large volume		% dose production compared to large volume
	152.04		152.04

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