Fig. 5

Single-cell and spatial transcriptomic landscapes of PScore. A Boxplots show PScore in different TME subtypes of TCGA and five other datasets, including GSE19234, GSE35640, GSE54467, GSE65904, and PRJEB23709. The x-axis is TME subtype, and the y-axis is PScore. B, D Dimensionality reduction plots and feature plots of PScore in two melanoma single-cell datasets. The same cell types in both datasets are colored with the same color. The change in the gray, blue, and cyan colors in the legend represents the change in PScore from low to high. C, E Violin plots and dot plots to visualize PScore in different cell types. The x-axis is the TME subtype, and the y-axis of the violin plot is PScore. The color of different cells is the same as the dimensionality reduction plots above. For dot plot, the changes in the blue, white, and red colors in the legend represent the change in PScore from low to high, and the size of the dot indicates the expression ratio. F, G Cell type annotation and corresponding level of PScore in spatial transcriptome data. The whole tissue plane is annotated into four cell types: macrophages (yellow), stromal cells (purple), melanoma cells (dark gray), and T/B cells (cyan). Blue, white, and purple indicate the magnitudes of PScore: blue, low; purple, high. H Violin plot to visualize comparison of PScore in different clusters consistent with (F). The x-axis is cell type, and the y-axis is PScore