Fig. 7

Summary of the present study. We constructed a comprehensive cellular landscape of human biventricular myocardium and blood from 6 end-stage heart patients with ARVC and 2 normal controls by using single-cell RNA sequencing. We identified the increased cell ratios of M1-macrophage, activated fibroblast, and myofibroblast in right ventricle with ARVC compared to normal control. The cell type-specific association with ARVC were also described. To sure robust science, we used two validation methods of the key findings of single-cell RNA sequencing data, including pathological staining in human heart with a larger sample size and drug intervention in an ARVC mouse model. ARVC, arrhythmogenic right ventricular cardiomyopathy